Publication (2016-2020)


Yu, J., L. E. Gimenez, C. C. Hernandez, Y. Wu, A. H. Wein, G. W. Han, K. McClary, S. R. Mittal, K. Burdsall, B. Stauch, L. Wu, S. N. Stevens, A. Peisley, S. Y. Williams, V. Chen, G. L. Millhauser, S. Zhao, R. D. Cone, and R. C. Stevens. Determination of the melanocortin-4 receptor structure identifies Ca2+ as a cofactor for ligand binding. Science. 2020;368(6489): 428-433.

Wu, F., L. Yang, K. Hang, M. Laursen, L. Wu, G. W. Han, Q. Ren, N. K. Roed, G. Lin, M. A. Hanson, H. Jiang, M. W. Wang, S. Reedtz-Runge, G. Song, and R. C. Stevens. Full-length human GLP-1 receptor structure without orthosteric ligands. Nat Commun. 2020;11(1):1272.;


Chen, X., Y. Xu, L. Qu, L. Wu, G. W. Han, Y. Guo, Y. Wu, Q. Zhou, Q. Sun, C. Chu, J. Yang, L. Yang, Q. Wang, S. Yuan, L. Wang, T. Hu, H. Tao, Y. Sun, Y. Song, L. Hu, Z. J. Liu, R. C. Stevens, S. Zhao, D. Wu, and G. Zhong. Molecular Mechanism for Ligand Recognition and Subtype Selectivity of alpha2C Adrenergic Receptor. Cell Rep. 2019;29(10):2936-2943.;

Claff, T., J. Yu, V. Blais, N. Patel, C. Martin, L. Wu, G. W. Han, B. J. Holleran, O. Van der Poorten, K. L. White, M. A. Hanson, P. Sarret, L. Gendron, V. Cherezov, V. Katritch, S. Ballet, Z. J. Liu, C. E. Muller, and R. C. Stevens. Elucidating the active delta-opioid receptor crystal structure with peptide and small-molecule agonists. Sci Adv. 2019;5(11):eaax9115.;

Laprairie, R. B., K. Vemuri, E. L. Stahl, A. Korde, J. H. Ho, T. W. Grim, T. Hua, Y. Wu, R. C. Stevens, Z. J. Liu, A. Makriyannis, and L. M. Bohn. Probing the CB1 Cannabinoid Receptor Binding Pocket with AM6538, a High-Affinity Irreversible Antagonist. Mol Pharmacol. 2019;96(5):619-28.;

Lu, Y., S. Qin, B. Zhang, A. Dai, X. Cai, M. Ma, Z. G. Gao, D. Yang, R. C. Stevens, K. A. Jacobson, M. W. Wang, and W. Shui. Accelerating the Throughput of Affinity Mass Spectrometry-Based Ligand Screening toward a G Protein-Coupled Receptor. Anal Chem. 2019;91(13):8162-9.;

Qu, L., Q. Zhou, Y. Xu, Y. Guo, X. Chen, D. Yao, G. W. Han, Z. J. Liu, R. C. Stevens, G. Zhong, D. Wu, and S. Zhao. Structural Basis of the Diversity of Adrenergic Receptors. Cell Rep. 2019;29(10):2929-35 e4.;

Xue, D., L. Ye, J. Zheng, Y. Wu, X. Zhang, Y. Xu, T. Li, R. C. Stevens, F. Xu, M. Zhuang, S. Zhao, F. Zhao, and H. Tao. The structure-based traceless specific fluorescence labeling of the smoothened receptor. Org Biomol Chem. 2019;17(25):6136-42.;

Zhao, S., B. Wu, and R. C. Stevens. Advancing Chemokine GPCR Structure Based Drug Discovery. Structure. 2019;27(3):405-8.;

Audet M, Stevens RC. Emerging structural biology of lipid G protein-coupled receptors. Protein Sci. 2019 Feb, 28(2):292-304.;

Audet M, White KL, Breton B, Zarzycka B, Han GW, Lu Y, Gati C, Batyuk A, Popov P, Velasquez J, Manahan D, Hu H, Weierstall U, Liu W, Shui W, Katritch V, Cherezov V, Hanson MA, Stevens RC. Crystal structure of misoprostol bound to the labor inducer prostaglandin E2 receptor. Nat Chem Biol. 2019 Jan, 15(1):11-17.;


Singla J, McClary KM, White KL, Alber F, Sali A, Stevens RC. Opportunities and Challenges in Building a Spatiotemporal Multi-scale Model of the Human Pancreatic β Cell. Cell. 2018 Mar 8, 173(1):11-19.;

Vass M, Kooistra AJ, Yang D, Stevens RC, Wang MW, de Graaf C. Chemical Diversity in the G Protein-Coupled Receptor Superfamily. Trends Pharmacol Sci. 2018 May, 39(5):494-512.;

Peng Y, McCorvy JD, Harpsøe K, Lansu K, Yuan S, Popov P, Qu L, Pu M, Che T, Nikolajsen LF, Huang XP, Wu Y, Shen L, Bjørn-Yoshimoto WE, Ding K, Wacker D, Han GW, Cheng J, Katritch V, Jensen AA, Hanson MA, Zhao S, Gloriam DE, Roth BL, Stevens RC, Liu ZJ. 5-HT2C Receptor Structures Reveal the Structural Basis of GPCR Polypharmacology. Cell. 2018 Feb 8, Pages 719–730.e14.;

White KL, Eddy MT, Gao ZG, Han GW, Lian T, Deary A, Patel N, Jacobson KA, Katritch V, Stevens RC. Structural Connection between Activation Microswitch and Allosteric Sodium Site in GPCR Signaling. Structure. 2018 Feb 6, 259–269.e5.;

Che T, Majumdar S, Zaidi SA, Ondachi P, McCorvy JD, Wang S, Mosier PD, Uprety R, Vardy E, Krumm BE, Han GW, Lee MY, Pardon E, Steyaert J, Huang XP, Strachan RT, Tribo AR, Pasternak GW, Carroll FI, Stevens RC, Cherezov V, Katritch V, Wacker D, Roth BL. Structure of the Nanobody-Stabilized Active State of the Kappa Opioid Receptor. Cell. 2018 Jan 11;172(1-2):55-67.e15.;

Zhang H, Qiao A, Yang L, Van Eps N, Frederiksen KS, Yang D, Dai A, Cai X, Zhang H, Yi C, Cao C, He L, Yang H, Lau J, Ernst OP, Hanson MA, Stevens RC, Wang MW, Reedtz-Runge S, Jiang H, Zhao Q, Wu B. Structure of the glucagon receptor in complex with a glucagon analogue. Nature. 2018 Jan 3;553(7686):106-110.;

Roach JJ, Sasano Y, Schmid CL, Zaidi S, Katritch V, Stevens RC, Bohn LM, Shenvi RA. Dynamic Strategic Bond Analysis Yields a Ten-Step Synthesis of 20-nor-Salvinorin A, a Potent κ-OR Agonist. ACS Cent Sci. 2017 Dec 27;3(12):1329-1336.;

Eddy MT, Lee MY, Gao ZG, White KL, Didenko T, Horst R, Audet M, Stanczak P, McClary KM, Han GW, Jacobson KA, Stevens RC, Wüthrich K. Allosteric Coupling of Drug Binding and Intracellular Signaling in the A2A Adenosine Receptor. Cell. 2018 Jan 11;172(1-2):68-80.e12.;


de Graaf C, Song G, Cao C, Zhao Q, Wang MW, Wu B, Stevens RC. Extending the Structural View of Class B GPCRs. Trends Biochem Sci. 2017 Dec;42(12):946-960.;

L. Ye, K. Ding, F. Zhao, X. Liu, Y. Wu, Y. Liu, D. Xue, F. Zhou, X. Zhang, R.C. Stevens, F. Xu, S. Zhao, H. Tao. A structurally guided dissection-then-evolution strategy for ligand optimization of smoothened receptor. Med. Chem. Commun., 2017, 8, 1332-13.;

X.E. Zhou, Y. He, P.W. de Waal, X. Gao, Y. Kang, N. Van Eps, Y. Yin, K. Pal, D. Goswami, T.A. White, A. Barty, N.R. Latorraca, H.N. Chapman, W.L. Hubbell, R.O. Dror, R.C. Stevens, V. Cherezov, V.V. Gurevich, P.R. Griffin, O.P. Ernst, K. Melcher, H.E. Xu ( 2017) Identification of Phosphorylation Codes for Arrestin Recruitment by G Protein-Coupled Receptors. Cell. 2017 Jul 27; 170(3):457-469.e13.;

D. Wacker, R.C. Stevens, B.L. Roth ( 2017) How Ligands Illuminate GPCR Molecular Pharmacology. Cell. 2017 Jul 27; 170(3):414-427.;

A. Ishchenko, D. Wacker, M. Kapoor, A. Zhang, G.W. Han, S. Basu, N. Patel, M. Messerschmidt, U. Weierstall, W. Liu, V. Katritch, B.L. Roth, R.C. Stevens, V. Cherezov (2017) Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody. Proc Natl Acad Sci U S A. 2017 Aug 1; 114(31):8223-8228.;

F. Wu, G. Song, C. de Graaf, R.C. Stevens ( 2017) Structure and function of peptide-binding G protein-coupled receptors. J Mol Biol. 2017 Aug 18; 429(17):2726-2745.;

T. Hua, K. Vemuri, S.P. Nikas, R.B. Laprarie, Y. Wu, L. Qu, M. Pu, A. Korde, S. Jiang, J.H. Ho, G.W. Han, K. Ding, X. Li, H. Liu, M.A. Hanson, S. Zhao, L.M. Bohn, A. Makriyannis, R.C. Stevens, Z.J. Liu (2017) Crystal structure of agonist-bound cannabinoid receptor CB1. Nature. 547(7664):468-471.;

Y. Zheng, G.W. Han, R. Abagyan, B. Wu, R.C. Stevens, V. Cherezov, I. Kufareva, T.M. Handel (2017) Structure of CC chemokine receptor 5 with a potent chemokine antagonist reveals mechanisms of chemokine recognition and molecular mimicry by HIV. Immunity. 25, 1005-1017.e5.;

Y. Ma, Y. Yue, Y. Ma, Q. Zhang, Q. Zhou, Y. Song, Y. Shen, X. Li, X. Ma, C. Li, M.A. Hanson, G.W. Han, E.A. Sickmier, G. Swaminath, S. Zhao, R.C. Stevens, L.A. Hu, W. Zhong, M. Zhang, F. Xu (2017) Structural basis for apelin control of the human apelin receptor. Structure. 25, 858-866.;

H. Zhang, A. Qiao, D. Yang, L. Yang, A. Dai, C. de Graaf, S. Reedtz-Runge, V. Dharmarajan, H. Zhang, G.W. Han, T.D. Grant, R.G. Sierra, U. Weierstall, G. Nelson, W. Liu, Y. Wu, L. Ma, X. Cai, G. Lin, X. Wu, Z. Geng, Y. Dong, G. Song, P.R. Griffin, J. Lau, V. Cherezov, H. Yang, M.A. Hanson, R.C. Stevens, Q. Zhao, H. Jiang, M.W. Wang, B. Wu (2017) Structure of the full-length glucagon class B G-protein-coupled receptor. Nature. 2017 May 17; [Epub ahead of print];

G. Song, D. Yang, Y. Wang, C. de Graaf, Q. Zhou, S. Jiang, K. Liu, X. Cai, A. Dai, G. Lin, D. Liu, F. Wu, Y. Wu, S. Zhao, L. Ye, G.W. Han, J. Lau, B. Wu, M.A. Hanson, Z.J. Liu, M.W. Wang, R.C. Stevens (2017) Human GLP-1 receptor transmembrane domain structure in complex with allosteric modulators. Nature. 2017 May 17; [Epub ahead of print];

X. Zhang, F. Zhao, Y. Wu, J. Yang, G.W. Han, S. Zhao, A. Ishchenko, L. Ye, X. Lin, K. Ding, V. Dharmarajan, P.R. Griffin, C. Gati, G. Nelson, M.S. Hunter, M.A. Hanson, V. Cherezov, R.C. Stevens, W. Tan, H. Tao, F. Xu (2017) Crystal structure of a multi-domain human smoothened receptor in complex with a super stabilizing ligand. Nat Commun. 2017 May 17; 8:15383;

M. Koh, F. Nasertorabi, G.W. Han, R.C. Stevens, P.G. Schultz (2017) Generation of an Orthogonal Protein-Protein Interface with a Noncanonical Amino Acid. J Am Chem Soc. 2017 Apr 26; 139(16):5728-5731;

H. Zhang, G.W. Han, A. Batyuk, A. Ishchenko, K.L. White, N. Patel, A. Sadybekov, B. Zamlynny, M.T. Rudd, K. Hollenstein, A. Tolstikova, T.A. White, M.S. Hunter, U. Weierstall, W. Liu, K. Babaoglu, E.L. Moore, R.D. Katz, J.M. Shipman, M. Garcia-Calvo, S. Sharma, P. Sheth, S.M. Soisson, R.C. Stevens, V. Katritch, V. Cherezov ( 2017) Structural basis for selectivity and diversity in angiotensin II receptors. Nature. 2017 Apr 20 544(7650):327-332;

Z. Zheng, X.P. Huang, T.J. Mangano, R. Zou, X. Chen, S. Zaidi, B.L. Roth, R.C. Stevens, V. Katritch (2017) Structure based discovery of new antagonist and biased agonist chemotypes for the Kappa Opioid Receptor J Med Chem. 2017 Mar 24.[Epub ahead of print];


Y. Zheng, L. Qin, N. V. Zacarías, H. de Vries, G. W. Han, M. Gustavsson, M. Dabros, C. Zhao, R. J. Cherney, P. Carter, D. Stamos, R. Abagyan, V. Cherezov, R. C. Stevens, A. P. IJzerman, L. H. Heitman, A. Tebben, I. Kufareva, T. M. Handel (2016) Structure of CC chemokine receptor 2 with orthosteric and allosteric antagonists Nature 540(7633): 458-461;

M. T. Eddy, T. Didenko, R. C. Stevens, and K. Wüthrich (2016) β 2-Adrenergic Receptor Conformational Response to Fusion Protein in the Third Intracellular Loop Structure : 2016 Nov 8 [Epub ahead of print];

T. Hua, K. Vemuri, M. Pu, L. Qu, G.W. Han, Y. Wu, S. Zhao, W. Shui, S. Li, A. Korde, R.B. Laprairie, E.L. Stahl, J.-H. Ho, N. Zvonok, H. Zhou, I. Kufareva, B. Wu, Q. Zhao, M.A. Hanson, L.M. Bohn, A. Makriyannis, R.C. Stevens, Z.-J. Liu ( 2016) Crystal structure of the human cannabinoid receptor CB 1 Cell 167(3): 750-762.e14, 20 October 2016; VIEW

S. Yuan, H.C. Chan, H. Vogel, S. Filipek, R.C. Stevens, K. Palczewski ( 2016) The Molecular Mechanism of P2Y 1 Receptor Activation Angew Chem Int Ed Engl. 55: 10331-10335;

X. Lv, J. Liu, Q. Shi, Q. Tan, D. Wu, J.J. Skinner, A.L. Walker, L. Zhao, X. Gu, N. Chen, L. Xue, P. Si, L. Zhang, Z. Wang, V. Katritch, Z.J. Liu, R.C. Stevens (2016) In vitro expression and analysis of the 826 human G protein-coupled receptors Protein Cell 7(5): 325-37;

X.E. Zhou, X. Gao, A. Barty, Y. Kang, Y. He, W. Liu, A. Ishchenko, T.A. White, O. Yefanov, G.W. Han, Q. Xu, P.W. de Waal, K.M. Suino-Powell, S. Boutet, G.J. Williams, M. Wang, D. Li, M. Caffrey, H.N. Chapman, J.C. Spence, P. Fromme, U. Weierstall, R.C. Stevens, V. Cherezov, K. Melcher, H.E. Xu (2016) X-ray laser diffraction structure determination of the rhodopsin-arrestin complex Sci. Data 3: 160021;

D. Yang, C. de Graaf, L. Yang, G. Song, A. Dai, X. Cai, Y. Feng, S. Reedtz-Runge, M.A. Hanson, H. Yang, H. Jiang, R.C. Stevens, M.W. Wang ( 2016) Structural determinants of binding the seven-transmembrane domain of the glucagon-like peptide-1 receptor J. Biol. Chem. 291: 12991-13004;

B.C. Bennett, M.D. Purdy, K.A. Baker, C. Acharya, W.E. McIntire, R.C. Stevens, Q. Zhang, A.L. Harris, R. Abagyan, M. Yeager (2016) An electrostatic mechanism for Ca2+-mediated regulation of gap junction channels Nat. Commun. 7: 8770;